Lepista sordida is a valuable edible mushroom rich in natural bioactive compounds.In the present study, a high-quality whole-genome of a domesticated strain of L.sordida was sequenced, revealing a 40.67 Mb genome in 13 contigs.Phylogenetic analysis revealed that L.
sordida is evolutionarily closely related to edible mushroom Lyophyllum decastes and Hypsizygus marmoreus.Heat stress has a significant effect on the yield and quality of mushrooms, but the molecular basis for here this is poorly understood in L.sordida.A label-free comparative proteomic analysis was performed under different heat stress conditions.The growth of L.
sordida mycelia was inhibited, and nuclear apoptosis occurred under heat stress.Ca2+ and MAPK signaling pathways were found to be involved in heat stress signal transduction.It is bar drain board hypothesized that the expression of various heat shock proteins plays a crucial role in the response to heat stress.In addition, the components of the ubiquitin-proteasome system and the thioredoxin system were upregulated, preventing the accumulation of misfolded proteins and possibly supporting the response to heat stress.In summary, these results provide a fundamental insight into the evolution and heat stress-responsive mechanisms in L.
sordida and may facilitate the breeding of heat-tolerant strains for artificial cultivation.